James_Madison_Lives
Member
- Joined
- Apr 11, 2010
- Messages
- 2,804
So throughout human history, 50 or a hundred years usually between major pandemic events, last biggie was Spanish Flu and they are coming every other year?
Boom. Fauci-Backed Wuhan Lab Did Gain-of-Function Monkeypox Research in February 2022
The editor of National Pulse, Raheem Kassam, was recently published in Newsweek.
Below: National Pulse editor Raheem Kassam
Via National Pulse
The Infamous Wuhan Lab Recently Assembled Monkeypox Strains Using Methods Flagged For Creating ‘Contagious Pathogens’.
BY NATALIE WINTERS MAY 22, 2022
"The Wuhan Institute of Virology assembled a monkeypox virus genome, allowing the virus to be identified through PCR tests, using a method researchers flagged for potentially creating a “contagious pathogen,” The National Pulse can reveal.
The Wuhan Institute of Virology assembled a monkeypox virus genome, allowing the virus to be identified through PCR tests, using a method researchers flagged for potentially creating a “contagious pathogen,” The National Pulse can reveal.
Monkey pox viruses – referred to as “MPXVs” in the paper – have strains that are “more pathogenic and [have] been reported to infect humans in various parts of the world.”
“Quantitative polymerase chain reaction (qPCR) is the gold standard for the detection of orthopoxvirus (including MPXV). For pan-orthopoxviruses detection, the E9L (DNA polymerase) gene has been shown to be an excellent target for qPCR assays. For MPXV detection, Li et al. reported that the C3L (complement-binding protein) gene could be used as the qPCR target for the MPXV Congo Basin strain,” explained the paper before noting that China lacked sufficient genetic information on the virus for PCR detection:
“Since MPXV infection has never been associated with an outbreak in China, the viral genomic material required for qPCR detection is unavailable. In this report, we employed dual-selective TAR to assemble a 55-kb MPXV genomic fragment that encompasses E9L and C3L, two valuable qPCR targets for detecting MPXV or other orthopoxviruses.”
“The primary purpose of assembling a fragment of the MPXV genome is to provide a nucleotide template for MPXV detection,” reiterated the study, which relied on the process of transformation-associated recombination (TAR) to isolate a genomic fragment of the monkeypox virus.
“As an efficient tool for assembling large DNA fragments up to 592 kb in length, TAR assembly has become essential for preparing infectious clones of large DNA/RNA viruses,” explained researchers.
The paper acknowledged that TAR “applied in virological research could also raise potential security concerns, especially when the assembled product contains a full set of genetic material that can be recovered into a contagious pathogen.”"
READ FULL ARTICLE AT NATIONAL PULSE…
Steve Hilton Investigative Report on Fauci-NIH Funding to Wuhan Lab for gain-of-function research, February 2, 2021 (View at Bitchute)
https://www.bitchute.com/video/k1AcLcjiJsxX/
The Hill: Senator Rand Paul Refers Fauci to Department of Justice for Lying to Congress About Funding Wuhan Gain-of-Function Research
Boom. Fauci-Backed Wuhan Lab Did Gain-of-Function Monkeypox Research in February 2022
The editor of National Pulse, Raheem Kassam, was recently published in Newsweek.
Below: National Pulse editor Raheem Kassam
Via National Pulse
The Infamous Wuhan Lab Recently Assembled Monkeypox Strains Using Methods Flagged For Creating ‘Contagious Pathogens’.
BY NATALIE WINTERS MAY 22, 2022
"The Wuhan Institute of Virology assembled a monkeypox virus genome, allowing the virus to be identified through PCR tests, using a method researchers flagged for potentially creating a “contagious pathogen,” The National Pulse can reveal.
The Wuhan Institute of Virology assembled a monkeypox virus genome, allowing the virus to be identified through PCR tests, using a method researchers flagged for potentially creating a “contagious pathogen,” The National Pulse can reveal.
Monkey pox viruses – referred to as “MPXVs” in the paper – have strains that are “more pathogenic and [have] been reported to infect humans in various parts of the world.”
“Quantitative polymerase chain reaction (qPCR) is the gold standard for the detection of orthopoxvirus (including MPXV). For pan-orthopoxviruses detection, the E9L (DNA polymerase) gene has been shown to be an excellent target for qPCR assays. For MPXV detection, Li et al. reported that the C3L (complement-binding protein) gene could be used as the qPCR target for the MPXV Congo Basin strain,” explained the paper before noting that China lacked sufficient genetic information on the virus for PCR detection:
“Since MPXV infection has never been associated with an outbreak in China, the viral genomic material required for qPCR detection is unavailable. In this report, we employed dual-selective TAR to assemble a 55-kb MPXV genomic fragment that encompasses E9L and C3L, two valuable qPCR targets for detecting MPXV or other orthopoxviruses.”
“The primary purpose of assembling a fragment of the MPXV genome is to provide a nucleotide template for MPXV detection,” reiterated the study, which relied on the process of transformation-associated recombination (TAR) to isolate a genomic fragment of the monkeypox virus.
“As an efficient tool for assembling large DNA fragments up to 592 kb in length, TAR assembly has become essential for preparing infectious clones of large DNA/RNA viruses,” explained researchers.
The paper acknowledged that TAR “applied in virological research could also raise potential security concerns, especially when the assembled product contains a full set of genetic material that can be recovered into a contagious pathogen.”"
READ FULL ARTICLE AT NATIONAL PULSE…
Steve Hilton Investigative Report on Fauci-NIH Funding to Wuhan Lab for gain-of-function research, February 2, 2021 (View at Bitchute)
https://www.bitchute.com/video/k1AcLcjiJsxX/
The Hill: Senator Rand Paul Refers Fauci to Department of Justice for Lying to Congress About Funding Wuhan Gain-of-Function Research
Last edited:
